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phosphorylated p erk  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology phosphorylated p erk
    Phosphorylated P Erk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2444 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/erk/p-ERK+1%2F2+Antibody/pm42788353-124-15-21
    Average 96 stars, based on 2444 article reviews
    phosphorylated p erk - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion.
    Article Snippet: Equal amounts of protein (20 μg, quantified by BCA (cat. no. PC0020; Beijing Solarbio Science & Technology Co., Ltd.) were separated by SDS‐PAGE (4‐20% gradient gel; cat. no. ET15420L; ACE Biotechnology) and transferred to 0.2 μm pore size PVDF membranes (cat. no. ISEQ00010; Merck Sharp & Dohme‐Hoddesdon). .. Following the blocking of nonspecific binding sites using 5% skimmed milk (cat. no. P0216; Beyotime Biotechnology) or bovine serum albumin (cat. no. NGP0028A; Beyotime Biotechnology) for 1 h at room temperature, the membranes were incubated with primary antibodies (incubation overnight at 4 ̊C) against SERPINE1 (cat. no. 13801‐1‐AP; Proteintech Group, Inc), GAPDH (cat. no. sc‐25778; Santa Cruz Biotechnology, Inc.), MCM3 (cat. no. PA5‐79646; Thermo Fisher Scientific, Inc.), phosphorylated (p‐)MCM3 (Ser112; cat. no. TA2362; Abmart Pharmaceutical Technology Co., Ltd.), ERK (cat. no. sc‐514302; Santa Cruz Biotechnology, Inc.), uPAR (cat. no. ab10379; Abcam), Histone H3 (cat. no. 4499), p‐p53 (Ser15; cat. no. 9284), p53 (cat. no. 2524), p‐SMAD3 (Ser423/425; cat. no. 9520), SMAD3 (cat. no. 9523), p‐Rb (Ser807/811; cat. no. 8516), Rb (cat. no. 9309), CyclinD1 (cat. no. 55506), CyclinE1 (cat. no. 20808), p21 (cat. no. 2947), p‐p38 (cat. no. 4511), p38 (cat. no. 8690), p‐ERK (cat. no. 4370), p‐JNK (cat. no. 9255), JNK (cat. no. 9252), p‐AKT (Ser473; cat. no. 4060), AKT (cat. no. 9272), HSP90α (cat. no. 4877) and MMP‐1 (cat. no. 54376) were from Cell Signaling Technology, Inc.) and subsequently incubated with the corresponding secondary antibodies (dilution 1:4,000) for 2 h at room temperature. .. The secondary antibodies used were horseradish peroxi‐ dase (HRP)‐conjugated goat anti‐rabbit (cat. no. ZB‐2301; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) and HRP‐conjugated goat anti‐mouse (cat. no. ZB‐2305; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.).

    Article Title: Ramalin Ameliorates Alzheimer's Disease Pathology by Targeting BACE1, HDAC6, and MAPK Pathways.
    Article Snippet: .. PVDF membranes were then incubated in a blocking buffer containing 5% nonfat dry milk at room temperature for 1 h. Following blocking, the PVDF membranes were incubated at 4◦C for overnight with primary antibodies against Ac-tubulin (Sigma), BACE1 (Cell Signaling Technology), β-actin (Invitrogen), caspase-1 (AdipoGen Life Sciences), COX2 (Abcam), ERK (Cell Signaling Technology), GFP (Santa Cruz Biotechnology), HDAC6 (Cell Signaling Technology), IL-1β (R&D Systems), iNOS (Santa Cruz Biotechnology), JNK (Cell Signaling Technology, Epitomics), LC3 (Novus Biologicals), NLRP3 (Cell Signaling Technology, ProSci), p-ERK (Cell Signaling Technology), p-JNK (Cell Signaling Technology), p-p38 (Cell Signaling Technology), p-TauS202, T205 (AT8; Invitrogen), p38 (Cell Signaling Technology), PHF-1 (In-house), Tau13 (BioLegend), and tubulin (Cell Signaling Technology). ..

    Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion
    Article Snippet: Equal amounts of protein (20 μ g, quantified by BCA (cat. no. PC0020; Beijing Solarbio Science & Technology Co., Ltd.) were separated by SDS-PAGE (4-20% gradient gel; cat. no. ET15420L; ACE Biotechnology) and transferred to 0.2 μ m pore size PVDF membranes (cat. no. ISEQ00010; Merck Sharp & Dohme-Hoddesdon). .. Following the blocking of nonspecific binding sites using 5% skimmed milk (cat. no. P0216; Beyotime Biotechnology) or bovine serum albumin (cat. no. NGP0028A; Beyotime Biotechnology) for 1 h at room temperature, the membranes were incubated with primary antibodies (incubation overnight at 4°C) against SERPINE1 (cat. no. 13801-1-AP; Proteintech Group, Inc), GAPDH (cat. no. sc-25778; Santa Cruz Biotechnology, Inc.), MCM3 (cat. no. PA5-79646; Thermo Fisher Scientific, Inc.), phosphorylated (p-)MCM3 (Ser112; cat. no. TA2362; Abmart Pharmaceutical Technology Co., Ltd.), ERK (cat. no. sc-514302; Santa Cruz Biotechnology, Inc.), uPAR (cat. no. ab10379; Abcam), Histone H3 (cat. no. 4499), p-p53 (Ser15; cat. no. 9284), p53 (cat. no. 2524), p-SMAD3 (Ser423/425; cat. no. 9520), SMAD3 (cat. no. 9523), p-Rb (Ser807/811; cat. no. 8516), Rb (cat. no. 9309), CyclinD1 (cat. no. 55506), CyclinE1 (cat. no. 20808), p21 (cat. no. 2947), p-p38 (cat. no. 4511), p38 (cat. no. 8690), p-ERK (cat. no. 4370), p-JNK (cat. no. 9255), JNK (cat. no. 9252), p-AKT (Ser473; cat. no. 4060), AKT (cat. no. 9272), HSP90α (cat. no. 4877) and MMP-1 (cat. no. 54376) were from Cell Signaling Technology, Inc.) and subsequently incubated with the corresponding secondary antibodies (dilution 1:4,000) for 2 h at room temperature. .. The secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-rabbit (cat. no. ZB-2301; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) and HRP-conjugated goat anti-mouse (cat. no. ZB-2305; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.).

    Binding Assay:

    Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion.
    Article Snippet: Equal amounts of protein (20 μg, quantified by BCA (cat. no. PC0020; Beijing Solarbio Science & Technology Co., Ltd.) were separated by SDS‐PAGE (4‐20% gradient gel; cat. no. ET15420L; ACE Biotechnology) and transferred to 0.2 μm pore size PVDF membranes (cat. no. ISEQ00010; Merck Sharp & Dohme‐Hoddesdon). .. Following the blocking of nonspecific binding sites using 5% skimmed milk (cat. no. P0216; Beyotime Biotechnology) or bovine serum albumin (cat. no. NGP0028A; Beyotime Biotechnology) for 1 h at room temperature, the membranes were incubated with primary antibodies (incubation overnight at 4 ̊C) against SERPINE1 (cat. no. 13801‐1‐AP; Proteintech Group, Inc), GAPDH (cat. no. sc‐25778; Santa Cruz Biotechnology, Inc.), MCM3 (cat. no. PA5‐79646; Thermo Fisher Scientific, Inc.), phosphorylated (p‐)MCM3 (Ser112; cat. no. TA2362; Abmart Pharmaceutical Technology Co., Ltd.), ERK (cat. no. sc‐514302; Santa Cruz Biotechnology, Inc.), uPAR (cat. no. ab10379; Abcam), Histone H3 (cat. no. 4499), p‐p53 (Ser15; cat. no. 9284), p53 (cat. no. 2524), p‐SMAD3 (Ser423/425; cat. no. 9520), SMAD3 (cat. no. 9523), p‐Rb (Ser807/811; cat. no. 8516), Rb (cat. no. 9309), CyclinD1 (cat. no. 55506), CyclinE1 (cat. no. 20808), p21 (cat. no. 2947), p‐p38 (cat. no. 4511), p38 (cat. no. 8690), p‐ERK (cat. no. 4370), p‐JNK (cat. no. 9255), JNK (cat. no. 9252), p‐AKT (Ser473; cat. no. 4060), AKT (cat. no. 9272), HSP90α (cat. no. 4877) and MMP‐1 (cat. no. 54376) were from Cell Signaling Technology, Inc.) and subsequently incubated with the corresponding secondary antibodies (dilution 1:4,000) for 2 h at room temperature. .. The secondary antibodies used were horseradish peroxi‐ dase (HRP)‐conjugated goat anti‐rabbit (cat. no. ZB‐2301; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) and HRP‐conjugated goat anti‐mouse (cat. no. ZB‐2305; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.).

    Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion
    Article Snippet: Equal amounts of protein (20 μ g, quantified by BCA (cat. no. PC0020; Beijing Solarbio Science & Technology Co., Ltd.) were separated by SDS-PAGE (4-20% gradient gel; cat. no. ET15420L; ACE Biotechnology) and transferred to 0.2 μ m pore size PVDF membranes (cat. no. ISEQ00010; Merck Sharp & Dohme-Hoddesdon). .. Following the blocking of nonspecific binding sites using 5% skimmed milk (cat. no. P0216; Beyotime Biotechnology) or bovine serum albumin (cat. no. NGP0028A; Beyotime Biotechnology) for 1 h at room temperature, the membranes were incubated with primary antibodies (incubation overnight at 4°C) against SERPINE1 (cat. no. 13801-1-AP; Proteintech Group, Inc), GAPDH (cat. no. sc-25778; Santa Cruz Biotechnology, Inc.), MCM3 (cat. no. PA5-79646; Thermo Fisher Scientific, Inc.), phosphorylated (p-)MCM3 (Ser112; cat. no. TA2362; Abmart Pharmaceutical Technology Co., Ltd.), ERK (cat. no. sc-514302; Santa Cruz Biotechnology, Inc.), uPAR (cat. no. ab10379; Abcam), Histone H3 (cat. no. 4499), p-p53 (Ser15; cat. no. 9284), p53 (cat. no. 2524), p-SMAD3 (Ser423/425; cat. no. 9520), SMAD3 (cat. no. 9523), p-Rb (Ser807/811; cat. no. 8516), Rb (cat. no. 9309), CyclinD1 (cat. no. 55506), CyclinE1 (cat. no. 20808), p21 (cat. no. 2947), p-p38 (cat. no. 4511), p38 (cat. no. 8690), p-ERK (cat. no. 4370), p-JNK (cat. no. 9255), JNK (cat. no. 9252), p-AKT (Ser473; cat. no. 4060), AKT (cat. no. 9272), HSP90α (cat. no. 4877) and MMP-1 (cat. no. 54376) were from Cell Signaling Technology, Inc.) and subsequently incubated with the corresponding secondary antibodies (dilution 1:4,000) for 2 h at room temperature. .. The secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-rabbit (cat. no. ZB-2301; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) and HRP-conjugated goat anti-mouse (cat. no. ZB-2305; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.).

    Incubation:

    Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion.
    Article Snippet: Equal amounts of protein (20 μg, quantified by BCA (cat. no. PC0020; Beijing Solarbio Science & Technology Co., Ltd.) were separated by SDS‐PAGE (4‐20% gradient gel; cat. no. ET15420L; ACE Biotechnology) and transferred to 0.2 μm pore size PVDF membranes (cat. no. ISEQ00010; Merck Sharp & Dohme‐Hoddesdon). .. Following the blocking of nonspecific binding sites using 5% skimmed milk (cat. no. P0216; Beyotime Biotechnology) or bovine serum albumin (cat. no. NGP0028A; Beyotime Biotechnology) for 1 h at room temperature, the membranes were incubated with primary antibodies (incubation overnight at 4 ̊C) against SERPINE1 (cat. no. 13801‐1‐AP; Proteintech Group, Inc), GAPDH (cat. no. sc‐25778; Santa Cruz Biotechnology, Inc.), MCM3 (cat. no. PA5‐79646; Thermo Fisher Scientific, Inc.), phosphorylated (p‐)MCM3 (Ser112; cat. no. TA2362; Abmart Pharmaceutical Technology Co., Ltd.), ERK (cat. no. sc‐514302; Santa Cruz Biotechnology, Inc.), uPAR (cat. no. ab10379; Abcam), Histone H3 (cat. no. 4499), p‐p53 (Ser15; cat. no. 9284), p53 (cat. no. 2524), p‐SMAD3 (Ser423/425; cat. no. 9520), SMAD3 (cat. no. 9523), p‐Rb (Ser807/811; cat. no. 8516), Rb (cat. no. 9309), CyclinD1 (cat. no. 55506), CyclinE1 (cat. no. 20808), p21 (cat. no. 2947), p‐p38 (cat. no. 4511), p38 (cat. no. 8690), p‐ERK (cat. no. 4370), p‐JNK (cat. no. 9255), JNK (cat. no. 9252), p‐AKT (Ser473; cat. no. 4060), AKT (cat. no. 9272), HSP90α (cat. no. 4877) and MMP‐1 (cat. no. 54376) were from Cell Signaling Technology, Inc.) and subsequently incubated with the corresponding secondary antibodies (dilution 1:4,000) for 2 h at room temperature. .. The secondary antibodies used were horseradish peroxi‐ dase (HRP)‐conjugated goat anti‐rabbit (cat. no. ZB‐2301; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) and HRP‐conjugated goat anti‐mouse (cat. no. ZB‐2305; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.).

    Article Title: Ramalin Ameliorates Alzheimer's Disease Pathology by Targeting BACE1, HDAC6, and MAPK Pathways.
    Article Snippet: .. PVDF membranes were then incubated in a blocking buffer containing 5% nonfat dry milk at room temperature for 1 h. Following blocking, the PVDF membranes were incubated at 4◦C for overnight with primary antibodies against Ac-tubulin (Sigma), BACE1 (Cell Signaling Technology), β-actin (Invitrogen), caspase-1 (AdipoGen Life Sciences), COX2 (Abcam), ERK (Cell Signaling Technology), GFP (Santa Cruz Biotechnology), HDAC6 (Cell Signaling Technology), IL-1β (R&D Systems), iNOS (Santa Cruz Biotechnology), JNK (Cell Signaling Technology, Epitomics), LC3 (Novus Biologicals), NLRP3 (Cell Signaling Technology, ProSci), p-ERK (Cell Signaling Technology), p-JNK (Cell Signaling Technology), p-p38 (Cell Signaling Technology), p-TauS202, T205 (AT8; Invitrogen), p38 (Cell Signaling Technology), PHF-1 (In-house), Tau13 (BioLegend), and tubulin (Cell Signaling Technology). ..

    Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion
    Article Snippet: Equal amounts of protein (20 μ g, quantified by BCA (cat. no. PC0020; Beijing Solarbio Science & Technology Co., Ltd.) were separated by SDS-PAGE (4-20% gradient gel; cat. no. ET15420L; ACE Biotechnology) and transferred to 0.2 μ m pore size PVDF membranes (cat. no. ISEQ00010; Merck Sharp & Dohme-Hoddesdon). .. Following the blocking of nonspecific binding sites using 5% skimmed milk (cat. no. P0216; Beyotime Biotechnology) or bovine serum albumin (cat. no. NGP0028A; Beyotime Biotechnology) for 1 h at room temperature, the membranes were incubated with primary antibodies (incubation overnight at 4°C) against SERPINE1 (cat. no. 13801-1-AP; Proteintech Group, Inc), GAPDH (cat. no. sc-25778; Santa Cruz Biotechnology, Inc.), MCM3 (cat. no. PA5-79646; Thermo Fisher Scientific, Inc.), phosphorylated (p-)MCM3 (Ser112; cat. no. TA2362; Abmart Pharmaceutical Technology Co., Ltd.), ERK (cat. no. sc-514302; Santa Cruz Biotechnology, Inc.), uPAR (cat. no. ab10379; Abcam), Histone H3 (cat. no. 4499), p-p53 (Ser15; cat. no. 9284), p53 (cat. no. 2524), p-SMAD3 (Ser423/425; cat. no. 9520), SMAD3 (cat. no. 9523), p-Rb (Ser807/811; cat. no. 8516), Rb (cat. no. 9309), CyclinD1 (cat. no. 55506), CyclinE1 (cat. no. 20808), p21 (cat. no. 2947), p-p38 (cat. no. 4511), p38 (cat. no. 8690), p-ERK (cat. no. 4370), p-JNK (cat. no. 9255), JNK (cat. no. 9252), p-AKT (Ser473; cat. no. 4060), AKT (cat. no. 9272), HSP90α (cat. no. 4877) and MMP-1 (cat. no. 54376) were from Cell Signaling Technology, Inc.) and subsequently incubated with the corresponding secondary antibodies (dilution 1:4,000) for 2 h at room temperature. .. The secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-rabbit (cat. no. ZB-2301; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) and HRP-conjugated goat anti-mouse (cat. no. ZB-2305; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.).

    Western Blot:

    Article Title: METTL1 interacts with XPO5 to modulate pre-miRNA export
    Article Snippet: .. Antibodies recognizing human XPO5 (Proteintech, #28628-1-AP; 1:2000), V5 (Proteintech, #14440-1-AP; 1:2000), streptavidin (Thermo Scientific, #S911), METTL1 (Proteintech, #14994-1-AP; 1:1000), ERK (Santa Cruz, SC-514302; 1:100), and p-ERK (Cell Signaling, #4370T; 1:1000) were used as primary antibodies for western blot analysis. .. Goat anti-rabbit IgG (whole molecule)-peroxidase antibody (Sigma, #A0545) and m-IgGκ BP-HRP (Santa Cruz Biotechnology, #sc-516102) were employed as secondary antibodies.



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    Image Search Results


    (A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), IRS1, EGFR, ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, ERα, Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.

    Journal: bioRxiv

    Article Title: Transcriptomic Analysis of the Newborn Mammary Gland Identifies Prenatal Ethanol-Induced Remodeling of Insulin Signaling and Enhanced Proliferative Programming

    doi: 10.64898/2026.09.22.753596

    Figure Lengend Snippet: (A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), IRS1, EGFR, ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, ERα, Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.

    Article Snippet: Proteins examined included IRS1(#3407), phospho-IRS1 (Ser612) (#3203), phospho-IRS1 (Tyr895) (#3070), phospho-Akt1 (Ser473) (#4060), ErbB3 (#12708), phospho-ERK1/2 (Thr202/Tyr204) (#9101) were purchased from Cell signaling Technology, EGFR (sc-03), IGF1R (sc-712), Akt1 (sc-5298), Erk2 (sc-1647), ERα (sc-8002), Sox2 (sc-398254), IGFBP5 (sc-515116) and β-Actin (sc-47778) were purchased from Santa Cruz Biotechnology.

    Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Analysis